Supplementary Information to : Phosphatidylinositol 4 , 5 - bisphosphate clusters act as molecular beacons for vesicle recruitment
نویسندگان
چکیده
1Department of Nanobiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany 2Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany 3Department of Tumor Immunology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands 4Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland 5Department of Theoretical and Computational Biophysics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany 6Institute for Organic and Biomolecular Chemistry, Georg-August-University Göttingen, Göttingen, Germany 7Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK
منابع مشابه
ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes.
At the trans-Golgi network, clathrin coats containing AP-1 adaptor complexes are formed in an ARF1-dependent manner, generating vesicles transporting cargo proteins to endosomes. The mechanism of site-specific targeting of AP-1 and the role of cargo are poorly understood. We have developed an in vitro assay to study the recruitment of purified AP-1 adaptors to chemically defined liposomes prese...
متن کاملPhosphatidylinositol 4,5-bisphosphate stimulates vesicle formation from liposomes by brain cytosol.
As a step toward the elucidation of mechanisms in vesicle budding, a cell-free assay that measures cytosol-induced vesicle generation from liposomes was established. This assay then was used to explore the role of phosphoinositides in vesicle formation. Liposomes incubated with brain cytosol in the presence of ATP and GTP massively generated small vesicles, as assessed both quantitatively and q...
متن کاملPhosphatidylinositol-(4,5)-bisphosphate regulates clathrin-coated pit initiation, stabilization, and size
Clathrin-mediated endocytosis (CME) is the major mechanism for internalization in mammalian cells. CME initiates by recruitment of adaptors and clathrin to form clathrin-coated pits (CCPs). Nearly half of nascent CCPs abort, whereas others are stabilized by unknown mechanisms and undergo further maturation before pinching off to form clathrin-coated vesicles (CCVs). Phosphatidylinositol-(4,5)-b...
متن کاملPlasmalemmal phosphatidylinositol-4,5-bisphosphate level regulates the releasable vesicle pool size in chromaffin cells.
During exocytosis, certain phospholipids may act as regulators of secretion. Here, we used several independent approaches to perturb the phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] level in bovine chromaffin cells to investigate how changes of plasmalemmal PI(4,5)P2 affect secretion. Membrane levels of PI(4,5)P2 were estimated by analyzing images of lawns of plasma membranes labeled with ...
متن کاملPhosphatidylinositol phosphate kinase type I gamma regulates dynamics of large dense-core vesicle fusion.
Phosphatidylinositol-4,5-bisphosphate was proposed to be an important regulator of large dense-core vesicle exocytosis from neuroendocrine tissues. Here, we have examined the kinetics of secretion in chromaffin cells from mice lacking phosphatidylinositol phosphate kinase type I gamma, the major neuronal phosphatidylinositol-4-phosphate 5-kinase. Absence of this enzyme caused a reduction of the...
متن کامل